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vivacell_graurotbuttonklein  Recent news

September 2016

Cooperation with Dhofar University (Sultanate of Oman)

 

May 2016

We were awarded the Stifterverband's "Innovative through Research" for our research activities.

 

April 2016

New Version of SimDerma including 30 important dermato-cosmetic parameters.

Read more here.

 

November 2015:

New Services in neurotoxicity and neuroprotection

Read more here.

 

October 2015:

SimDerma - new screening platform for cosmetic ingredients.

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Read more here.

 

Services in Oral Care.

Read more here.

 

September 2015:

Please have a look at our new services in miRNA research.

Read more here.

 


 

New publications by VivaCell:

 

"Poly(I:C) increases the expression of mPGES-1 and COX-2 in rat primary
microglia."

in J Neuroinflamm. 2016 Jan

 

"Antimalarial Drug Artemether Inhibits Neuroinflammation in BV2 Microglia
Through Nrf2-Dependent Mechanisms."

in Mol Neurobiol. 2015 Nov

 

"microRNA-26a modulates inflammatory response induced by toll-like receptor 4 stimulation in microglia."

in J Neurochem, 2015 Sep

 

"Anti-inflammatory effects of 5-HT3 receptor antagonists in interleukin-1beta stimulated primary human chondrocytes."
in Int Immunopharmacol. 2014 Jun

"Histone deacetylase inhibitors valproic acid and sodium butyrate enhance prostaglandins release in lipopolysaccharide-activated primary microglia."
Neuroscience. 2014 Jan 28

 

 

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vivacell_graurotbuttonklein  Opinions from our customers:

p-pascoe-naturmedizin

Dr. G. Weiss (PASCOE pharmazeutische Praeparate GmbH):

:

"During our well going cooperation over several years, we learned to appreciate VivaCell as a reliable and competent co-operation partner in pre-clinical research. Therefore we are looking forward to continue our successful partnership."  

 

 

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opinions...

 

 

Phytodrug / Nutraceuticals
Immunomodulation
Cardiovascular inflammation
Oncology / Angiogenesis
Oral care
Central Nervous System
Microglia activation studies
Electrophysiological. Meas.
Dermatology / Cosmetics
Muscle
HIV-1 / AIDS
Diabetes
ADME
Permeability Studies
Toxicology
Urinary / Prostata
Animal health
Additional Services
Quality Certificate
SimDerma / Cosmetics
Neurotox testing / Protection
miRNA
Services in Oral Care

 

We are awarded for our R&D activities by the Stifterverband:

 

forschung_und_entwicklung_2016_web

 

 


 

We are proud members of:

 

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    koop-phyto-logo

ga 

 

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VivaCell Quality-Certificate:

 

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ONCOLOGY / ANGIOGENESIS


At VivaCell, we have adapted specific protocols “in vitro” to study the anti-tumoural activity of phytomedicines and nutraceuticals. More than 100 parameters including cellular, molecular and biochemical techniques are offered to our client, and by using such parameters we run projects adapted to each phytodrug and tailored to customer’s needs.

 

In vitro testing methods include proliferation inhibition assays using human tumour cell lines, as well as clonogenic assays with human tumor xenografts, human tumor cell lines, and hematopoetic stem cells. More than 350 solid tumor models have been established in serial passage as well as 20 permanent tumor cell lines (co-operation). In addition, a panel of 60 cell lines, commonly used in cancer research such as A549, MCF-7, Caco-2, HT29 or PC3, are also available. Cancer cell lines overexpressing the MDR1 gene are also available for drug research.


In vivo assays include subcutaneous models, orthotopic models with human xenografts, murine tumour models (co-operation) and models of topic co-carcinogenesis inducing papilloma-like skin tumours in athymic mice.


Angiogenesis(e.g. VEGF, angiopoietin I and II, nephrin, i.e.). In vitro3D assays (spheroids), HUVECs and Matrigel Basement Membrane Matrix, In vivocorneal neovascularization assays, 3D assays (spheroids injected s.c.),

 

Parameters:
· Proliferation, XTT assays, determination of DNA content by FACS analyses
· Cytokines (e.g. IL-6), growth factors (e.g. EGF) etc.
· Cell cycle analyses, cytotoxicity by measuring PI staining 

· Determination of DNA fragmentation (apoptosis) by TUNEL
· Transmembrane potential of the mitochondria, calcium and pH mobilisation
· Reactive oxygen species (ROS)
· Fas clustering and signalling in type I and II cells for apoptosis
Caspases (3, 7, 8, 6 and 9) activity by spectrofluorometry and western blots
· Bcl-2, Bcl-X, Bax, Bid and cytoplasmic cytochrome C by western blots and PCR.
· Apoptosis and cell cycle analyses in transfected MDR (pgp-1) cell lines
· Phosphatidyl serine expression at the cell surface by Anexin V binding.
· Integrity of tubulin and actin by fluorescence microscopy

· In vitro assays for tubulin polimerization.
· Cyclin activities by IP and Western blots
· Activation of Transcription Factors
· Signalling studies MAPKs, IKK, ie..
· Determination of Telomerase activity in HeLa cells by Telomerase PCR ELISA
· Customized “knock out” cell lines for specific genes
· DNA microarrays for cancer-related genes in tumoural cell lines

Other specific cellular and molecular techniques are available upon request.

 

 

 

 

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